Interaction study of Glutamic Acid with double-stranded calf thymus DNA by using Cyclic Voltammetric, Ultraviolet-visible and Fluorescence spectroscopy

Amit K Harit, Ratnesh Das, Priyanka Bose


Interaction of glutamic acid (Glu) was studied with double-stranded (ds) calf thymus DNA (CT-DNA) by cyclic voltammetry (CV) then obtained results were further correlated by ultraviolet-visible (UV-Vis) and fluorescence spectroscopies. Calculated binding constant from all techniques was very close to each other and it is around 3.54× 103 M L-1. Further binding sites were also calculated and it is near to 1, which indicates appropriate binding of Glu with CT-DNA. The result shows Glu binds in groove modes of DNA. Further binding free energy (G) of the complex was also calculated and it is -4.76 kCal M-1. In our work a correlative intractability pattern for Glu with DNA has been identified. 


Glutamic Acid; calf thymus DNA; Intercalating binding; Binding Constant

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K. Tao, A. Levin, L. Adler-Abramovichab E. Gazit, “Fmoc-modified amino acids and short peptides: simple bio-inspired building blocks for the fabrication of functional materials”, Chem. Soc. Rev. 45, 3935-3953 (2016).

S.K. Paul, M.M. Goldar, M. Yakura, Y. Oowatari, M. Kawamukai, “Glutamyl tRNA Synthetases and Glutamic Acid Induce Sexual Differentiation of Schizosaccharomyces pombe”., Biosci. Biotechnol. Biochem. 73, 1339-1347 (2009).

V.A. Najjar, “The Biological Effect of Glutamic Acid and Its Derivatives”, Mole. Cellul. biochem. 38, 167-170 (1981).

M. Watford, “Glutamine and glutamate: Nonessential or essential amino acids?”, Animal Nutrition. 1, 119–122 (2015).

S. Yamaguchi, K. Ninomiya, “Umami and food palatability”, J. of Nutr. 4, 246-251 (2000).

S. Fuke, T. Shimizu, “Sensory and preference aspects of umami”, Trends in F.ood Sci. Tech. 4, 246-251 (1993).

T. Populin, S. Moret, S. Truant, L.S. Conte, “A survey on the presence of free glutamic acid in foodstuffs, with and without added monosodium glutamate”, Food Chem. 104 1712-1717 (2007).

F. Bellisle, “Glutamate and the umami taste. Sensory, metabolic, nutritional and behavioural considerations. A review of the literature published in the last 10 years”. Neurosci. Biobehav. Rev. 23, 423-438 (1999).

T. Arakawa, K. Tsumoto, Y. Kita, B. Chang, D. Ejima, “Biotechnology applications of amino acids in protein purification and formulations”, Rev. Art. Amino Acids. 33, 587-605 (2007).

A.K. Mandal, S. Samaddar, R. Banerjee, S. Lahiri, A. Bhattacharyya, S. Roy, “Glutamate Counteracts the Denaturing Effect of Urea through Its Effect on the Denatured State”, J. Bio. Chem. 278, 36077-36084(2003).

Y. Akiba, C. Watanabe, M. Mizumori, J. D. Kaunitz, “Luminal L-glutamate enhances duodenal mucosal defense mechanisms via multiple glutamate receptors in rats”, A. J. Physio.: Gastro. and Liver Physio. 297, G781-G791 (2009).

T. Tsurugizawa, A. Uematsu, E. Nakamura, Hasumura, M. Hirota, “Mechanisms of neural response to gastrointestinal nutritive stimuli”, Gastroenterology. 37, 262-273 (2009).

K. Wachirasiri, S. Wanlapa, D. Uttapap, V. Rungsardthong, “Use of amino acids as a phosphate alternative and their effects on quality of frozen white shrimps (Penaeus vanamei)”, LWT - Food Science and Technology. 69, 303-311(2016).

S. Yamamoto, M. Tomoe, K. Toyama, M. Kawai, H. Uneyama, “Can dietary supplementation of monosodium glutamate improve the health of the elderly?”, A. J. Clin. Nutr. 90, S844-S849 (2009).

J. Rhodes, A.C. Titherley, J. A. Norman, R. Wood, D.W. Lord, “A survey of the monosodium glutamate content of foods and an estimation of the dietary intake of monosodium glutamate”, Food Addit. Contam. 8, 663-672 (1991).

Y. Zhousheng, “Electrochemical behaviour of gallic acid interaction with DNA and detection of damage to DNA”, J. Ele.Ana. Chem. 624, 91-96 (2008).

T. Bivera, “Use of UV-Vis Spectrometry to Gain Information on the Mode of Binding of Small Molecules to DNAs and RNAs”, Appl. Spectro. Rev. 47, 272-325 (2012).

A. Mehdinia, S.H. Kazemi, S.Z. Bathaie, A. Alizadeh, M. Shamsipur, M. Fazlollah, “Electrochemical DNA nano-biosensor for the study of spermidine–DNA interaction”, J. Phar. Bio. 49, 587-593 (2009).

J. Szejtli, “Past, present and future of cyclodextrin research”, Pure Appl. Chem. 76, 1825-1845 (2004).

A. Imran, A.W. Waseem, S. Kishwar, W. Diana, “Syntheses, DNA Binding and Anticancer Profiles of L-Glutamic Acid Ligand and its Copper(II) and Ruthenium(III) Complexes”, Med. Chem. 9, 11-21 (2013).

M.A. Husain, Z. Yaseen, S.U. Rehman, T. Sarwar, M. Tabish, “Naproxen intercalates with DNA and causes photocleavage through ROS generation”, FEBS J. 280, 6569-6580 (2013).

E. Jin, X. Bian, X. Lu, C. Wang, “Fabrication of multiwalled carbon nanotubes/polypyrrole/Prussian blue ternary composite nanofibers and their application for enzymeless hydrogen peroxide detection”, J. of Materials Science. 47, 4326–4331 (2012).

I. Khan, U. J. Pandit, S. Wankar, R. Das, S. N. Limaye; “Fabrication of electrochemical nanosensor based on polyaniline film-coated AgNP-MWCNT-modified GCE and its application for trace analysis of fenitrothion”, Ionics. 23, 1293–1308 (2017).

Ahmed M. Abdul-Lettif, “Determination of diffusion coefficients in Au/Ni thin filmsby Auger electron spectroscopy”, Phys. stat. sol. (a) 201, 2063–2066 (2004).

T. Sarwar, M.A. Husain, S.U. Rehman, H.M. Ishqi, M. Tabish, “Multi-spectroscopic and molecular modelling studies on the interaction of esculetin with calf thymus DNA”, Mol. Bio. Syst. 11, 522-531 (2015).

M.L. Yola, N. Ozaltin, “Electrochemical studies on the interaction of an antibacterial drug nitrofuranation with DNA”, J. Eletanal. Chem. 653, 56-60 (2011).

X. Hua , Y. F. Zhang, J. H. Chen, “Studies of the interaction between Aloe-emodin and DNA and preparation of DNA biosensor for detection of PML-RARα fusion gene in acute promyelocytic leukemia”, Talanta. 74, 944-950 (2008).

J.A. War, S.K. Srivastava, S.D. Srivastava, “Synthesis and DNA-binding study of imidazole linked thiazolidinone derivatives”, Luminescence. 32, 104–113 (2017).

C.V. Kumar, E.H. Punzalan, W.B. Tan, “Adenine-thymine base pair recognition by an anthryl probe from the DNA minor groove”, Tetrahedron. 56, 7027–40 (2000).

L. Fotouhi, R. Tabatabaee, “A study of the interaction tyrosin and DNA using voltammetry and spectroscopy methods”, Spectrochim, Acta Part A: Mol, Biomol. Spectro. 121 152-156 (2014).

G.A. Holdgate, W.H.J. Ward, “Measurements of binding thermodynamics in drug discovery”, Drug Dis. Today. 10, 1543-1550 (2005)


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